Development of a set of oligonucleotides for the identification of the Sakhalin sturgeon Acipenser mikadoi Hilgendorf, 1892 by PCR

Sturgeons (family Acipenseridae) are valuable commercial fish and aquaculture resources. The Acipenser genus includes about 20 species, 12 of which recorded for the Russian territory. One rarest is Sakhalin sturgeon A. mikadoi Hilgendorf, 1892. At present, its population size significantly decreased species close to extinction. Natural populations have survived in Tumnin River Khabarovsk Region Viakhtu Region. Vihtu Due small numbers rare endangered noninvasive approaches, including use DNA from environment, appear be most suitable monitoring their genetic diversity. method well established this area has been successfully tested several sturgeons. This paper presents results development primers specific mitochondrial A . matrix 15 sequences complete genomes 3 whose ranges may overlap - (KX276658), dauricus (KJ402277) schrenckii (MH973728- MH973734, KX276660, KX276659, KF150287, KC905169, KC820796) was generated. medirostris (NC_028405), genetically closest sturgeon, also included analysis. sliding window algorithm applied sequence matrix. graph showing distribution divergence values along genome plotted based on analysis Fig. 1. Based matrices individual fragments ( 16S rRNA, COI , ND1 D-loop ), a search species-specific performed DECIPHER program. Primer-BLAST run each pair primers. Taxa Actinopterygii Acipenseridae were used as references separately different runs. In addition, checked using local reference representatives primer evaluation summarized table Table As result, combination pairs developed basis seems successful error-free identification sturgeon.